Hepcidin-25的作用機(jī)制及在炎癥、心血管與腫瘤等動(dòng)物模型中的應(yīng)用

Hepcidin-25是一種由25個(gè)氨基酸組成的肽類激素，在其肽鏈中存在著一條金屬結(jié)合基序，并在脊椎動(dòng)物鐵代謝調(diào)控中發(fā)揮核心作用¹。作為鐵穩(wěn)態(tài)的"主調(diào)控因子"，它通過抑制腸道鐵吸收和巨噬細(xì)胞鐵釋放來調(diào)控循環(huán)鐵的水平²。Hepcidin-25常常作為疾病模型中的分析指標(biāo)或者生物標(biāo)志分子。例如在健康狀態(tài)下，大鼠血漿中Hepcidin-25的平均濃度為63.0 ng/mL，而在脂多糖刺激后顯著升高至218.2 ng/mL，表明Hepcidin-25與炎癥反應(yīng)高度相關(guān)³。在心血管領(lǐng)域，研究發(fā)現(xiàn)Hepcidin-25與動(dòng)脈僵硬度存在關(guān)聯(lián)⁴。Hepcidin-25還可調(diào)節(jié)鐵代謝，Hepcidin-25與其受體鐵輸出蛋白結(jié)合后，可抑制腸道鐵吸收以及肝細(xì)胞和巨噬細(xì)胞的鐵外排。檢測(cè)Hepcidin-25可以預(yù)測(cè)機(jī)體的鐵代謝狀態(tài)，并有助于研究貧血和某些以貧血為特征的動(dòng)物疾病模型，例如慢性腎臟�。–KD）、糖尿病相關(guān)的慢性腎病、炎癥性腸病血色病等動(dòng)物模型⁵。Hepcidin-25的升高還可用于監(jiān)測(cè)腫瘤的發(fā)生發(fā)展，例如Hepcidin-25與肝細(xì)胞癌的腎臟轉(zhuǎn)移有關(guān)⁶，并且Hepcidin-25還可作為非侵入性乳腺癌預(yù)測(cè)工具。目前針對(duì)Hepcidin-25的檢測(cè)手段包括LC-MS/MS法、ELISA檢測(cè)試劑盒等技術(shù)。Hepcidin-25還具有其它的生物學(xué)應(yīng)用例如抗菌，有研究表明Hepcidin-25的水平在細(xì)菌感染后會(huì)明顯上調(diào)，表明其與機(jī)體的主動(dòng)防御有關(guān)。而在體外實(shí)驗(yàn)中，Hepcidin-25及其衍生物Hepcidin-20對(duì)多種病原微生物表現(xiàn)出抑制活性⁷。
 
參考文獻(xiàn)及鳴謝
(1) Mead, M. K.; Claus, M.; Litton, E.; Smart, L.; Raisis, A.; Rossi, G.; Trengove, R. D.; Gummer, J. P. A. Identification of The Canidae Iron Regulatory Hormone Hepcidin. Scientific reports 2019, 9 (1), 19400. DOI: 10.1038/s41598-019-55009-w  From NLM.
(2) Kesharwani, P.; Dash, D.; Koiri, R. K. Deciphering the role of hepcidin in iron metabolism and anemia management. Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) 2025, 87, 127591. DOI: 10.1016/j.jtemb.2025.127591  From NLM.
(3) Sakamoto, S.; Kirinashizawa, M.; Mohara, Y.; Watanabe, Y. Generation and characterization of monoclonal antibodies against mature hepcidin and its application to neutralization and quantitative alteration assay. Bioscience, biotechnology, and biochemistry 2021, 85 (2), 340-350. DOI: 10.1093/bbb/zbaa013  From NLM.
(4) Wang, X.; Sheng, L.; Ye, P.; Cao, R.; Yang, X.; Xiao, W.; Zhang, Y.; Bai, Y.; Wu, H. The association between Hepcidin and arterial stiffness in a community-dwelling population. Lipids in health and disease 2018, 17 (1), 244. DOI: 10.1186/s12944-018-0866-6  From NLM.
(5) Ueda, N.; Takasawa, K. J. C. m. c. Role of hepcidin-25 in chronic kidney disease: anemia and beyond. 2017, 24 (14), 1417-1452.
(6) Kamai, T.; Tomosugi, N.; Abe, H.; Arai, K.; Yoshida, K.-I. J. B. c. Increased serum hepcidin-25 level and increased tumor expression of hepcidin mRNA are associated with metastasis of renal cell carcinoma. 2009, 9 (1), 270.
(7) Maisetta, G.; Petruzzelli, R.; Brancatisano, F. L.; Esin, S.; Vitali, A.; Campa, M.; Batoni, G. J. P. Antimicrobial activity of human hepcidin 20 and 25 against clinically relevant bacterial strains: effect of copper and acidic pH. 2010, 31 (11), 1995-2002.