Amphotericin B作為靶向細胞膜多效抗生素在抗感染與免疫激活中的應用
Amphotericin B（AMB，兩性霉素B，AbMole，M5415）是一種大環(huán)多烯類抗生素，其能夠與細胞膜中的特定甾醇結合，導致膜通透性改變、離子泄漏，最終引發(fā)細胞功能紊亂[1]。在抗菌方面，Amphotericin B通過上述結合，破壞真菌膜完整性并發(fā)揮抗真菌作用[2]。此外，Amphotericin B（AMB，兩性霉素B，AbMole，M5415）在HT-29人結腸腺癌細胞和正常結腸上皮細胞中能通過氧化損傷途徑影響細胞活性[3]。此外，在NK細胞系和原代NK細胞的研究中發(fā)現，Amphotericin B能增強效應細胞與靶細胞的結合，從而提升NK細胞的殺傷能力[4]。在動物模型研究中，Amphotericin B（CAS No.：1397-89-3）展現出多方面的應用價值。例如在兔肺感染模型中，Amphotericin B有效抑制了真菌感染，且避免了傳統抑制劑的副作用[5]。
Anticancer Res. 2021 May;41(5):2257-2275.
德國漢堡埃彭多夫大學、德國羅斯托克大學的科研人員在該文章中使用了AbMole的Amphotericin B（AMB，兩性霉素B，AbMole，M5415）科研人員在上述研究中系統探討了PI3K/AKT/mTOR信號通路在結直腸癌中的作用。研究發(fā)現，聯合使用AKT抑制劑MK2206 和mTOR抑制劑RAD001（Everolimus）對結直腸癌（CRC）細胞系和PDO腫瘤類器官具有顯著的協同抑制作用。并且體內實驗進一步證實聯合處理能有效抑制結直腸癌異種移植瘤的生長。Amphotericin B作為抗真菌藥物，在組織樣本處理和細胞培養(yǎng)過程中用于防止真菌污染，保護樣本的完整性和活性。
*本文所述產品僅供科研使用
參考文獻及鳴謝
[1] Guo, Z.; Zhang, H.; Jingele, X.; et al. Stanniocalcin 2 Promotes Neuronal Differentiation in Neural Stem/Progenitor Cells of the Mouse Subventricular Zone Through Activation of AKT Pathway. Stem cells and development 2024, 33 (19-20), 551-561.
[2] Baghirova, A. A.; Kasumov, K. M. Antifungal Macrocycle Antibiotic Amphotericin B-Its Present and Future. Multidisciplinary Perspective for the Use in the Medical Practice. Biochemistry (Moscow) Supplement. Series B, Biomedical chemistry 2022, 16 (1), 1-12.
[3] Grela, E.; Piet, M.; Luchowski, R.; et al. Imaging of human cells exposed to an antifungal antibiotic amphotericin B reveals the mechanisms associated with the drug toxicity and cell defence. Scientific reports 2018, 8 (1), 14067.
[4] Kim, N.; Choi, J. W.; Park, H. R.; et al. Amphotericin B, an Anti-Fungal Medication, Directly Increases the Cytotoxicity of NK Cells. International journal of molecular sciences 2017, 18 (6).
[5] Wasan, K. M. Development of an Oral Amphotericin B Formulation as an Alternative Approach to Parenteral Amphotericin B Administration in the Treatment of Blood-Borne Fungal Infections. Current pharmaceutical design 2020, 26 (14), 1521-1523.
Anticancer Res. 2021 May;41(5):2257-2275.
德國漢堡埃彭多夫大學、德國羅斯托克大學的科研人員在該文章中使用了AbMole的Amphotericin B（AMB，兩性霉素B，AbMole，M5415）科研人員在上述研究中系統探討了PI3K/AKT/mTOR信號通路在結直腸癌中的作用。研究發(fā)現，聯合使用AKT抑制劑MK2206 和mTOR抑制劑RAD001（Everolimus）對結直腸癌（CRC）細胞系和PDO腫瘤類器官具有顯著的協同抑制作用。并且體內實驗進一步證實聯合處理能有效抑制結直腸癌異種移植瘤的生長。Amphotericin B作為抗真菌藥物，在組織樣本處理和細胞培養(yǎng)過程中用于防止真菌污染，保護樣本的完整性和活性。
Sensitivity of 3D cell cultures of primary colorectal carcinoma (CRC) samples to inhibition of AKT serine/threonine kinase (AKT) and mammalian target of rapamycin (mTOR).
*本文所述產品僅供科研使用
參考文獻及鳴謝
[1] Guo, Z.; Zhang, H.; Jingele, X.; et al. Stanniocalcin 2 Promotes Neuronal Differentiation in Neural Stem/Progenitor Cells of the Mouse Subventricular Zone Through Activation of AKT Pathway. Stem cells and development 2024, 33 (19-20), 551-561.
[2] Baghirova, A. A.; Kasumov, K. M. Antifungal Macrocycle Antibiotic Amphotericin B-Its Present and Future. Multidisciplinary Perspective for the Use in the Medical Practice. Biochemistry (Moscow) Supplement. Series B, Biomedical chemistry 2022, 16 (1), 1-12.
[3] Grela, E.; Piet, M.; Luchowski, R.; et al. Imaging of human cells exposed to an antifungal antibiotic amphotericin B reveals the mechanisms associated with the drug toxicity and cell defence. Scientific reports 2018, 8 (1), 14067.
[4] Kim, N.; Choi, J. W.; Park, H. R.; et al. Amphotericin B, an Anti-Fungal Medication, Directly Increases the Cytotoxicity of NK Cells. International journal of molecular sciences 2017, 18 (6).
[5] Wasan, K. M. Development of an Oral Amphotericin B Formulation as an Alternative Approach to Parenteral Amphotericin B Administration in the Treatment of Blood-Borne Fungal Infections. Current pharmaceutical design 2020, 26 (14), 1521-1523.
