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  • 6848 次 一年4篇Cell一作論文 大牛分享了10條科研經(jīng)驗(yàn) 2016-2-25
    羅納德·韋爾博士（Ronald D. Vale）是加州大學(xué)舊金山分校（UCSF）的細(xì)胞與分子藥理學(xué)教授。研究方向是驅(qū)動(dòng)蛋白和動(dòng)力蛋白分子馬達(dá)。他創(chuàng)造了一個(gè)科研界的世界記錄：一年內(nèi)作為第一作者發(fā) 4 篇高

  • 7886 次 Wheaton細(xì)胞培養(yǎng)系列CELLine生物反應(yīng)器工作原理及特點(diǎn) 2015-5-27
    Wheaton的CELLine生物反應(yīng)器采用細(xì)胞室與培養(yǎng)基室半透膜分離培養(yǎng)技術(shù)，突破了傳統(tǒng)細(xì)胞培養(yǎng)的空氣，營(yíng)養(yǎng)物質(zhì)，代謝抑制因子對(duì)細(xì)胞生長(zhǎng)的制約。最大程度的模擬了細(xì)胞在有機(jī)體的生長(zhǎng)環(huán)境。實(shí)現(xiàn)了高

  • 6463 次 人全血Foxp3實(shí)驗(yàn)步驟 2014-12-3
    需要試劑 HumanRegulatoryTCellWholeBloodStainingKit（#88-8996-40） 包含組分： (1)、1XRBCLysisBuffer:200mL (2)、FlowCytometryStainingBuffer:600mL (3)、Fixation/PermeabilizationCon

  • 3211 次 Cell-Based ELISA簡(jiǎn)介以及應(yīng)用 2014-9-11
    1、Cell-Based ELISA 的優(yōu)點(diǎn)： Cell-Based ELISA（基于細(xì)胞的 ELISA）是一種全新的ELISA技術(shù)，有兩個(gè)最突出的優(yōu)點(diǎn)：1.1不需要抽提蛋白、包被微孔板：細(xì)胞直接在微孔板里培養(yǎng)，待檢測(cè)的時(shí)候，將細(xì)

  • 10348 次 血細(xì)胞形態(tài)學(xué)檢查的重要意義 2014-6-28
    血液一般檢驗(yàn)取材容易、檢測(cè)便捷，仍然是篩檢疾病、遴選其他實(shí)驗(yàn)檢查的首要程序。血細(xì)胞計(jì)數(shù)及血液形態(tài)學(xué)檢查是血液檢驗(yàn)項(xiàng)目中最基礎(chǔ)、最常用的檢驗(yàn)。血細(xì)胞形態(tài)學(xué)檢驗(yàn)是檢驗(yàn)醫(yī)學(xué)的基礎(chǔ)和核心，

  • 5766 次 T Cell Receptor 信號(hào)通路圖 2012-7-23
    The T Cell Receptor plays a key role in the immune system. The specificity of the receptor is governed by the binding site formed from the mature alpha and beta chains (shown here)

  • 4660 次 B Cell Receptor 信號(hào)通路圖 2012-7-13
    B cells produce immunoglobulins (Ig, antibodies) that specifically bind antigen molecules. B cells first produce a membrane-bound form of immunoglobulin, the B cell receptor, as pa

  • 2529 次 3H-Thymidine Uptake by Cultured Cells 2012-6-20
    Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chr

  • 1769 次 Fluorescent Staining of Cells 2012-6-20
    1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2. PBS, solution A. Procedure:1. Fix a

  • 3441 次 Culture of human venous smooth muscle cells 2012-5-23
    Culture of human venous smooth muscle cells1.Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared according to the method described f

  • 3296 次 Isolation of human prostatic smooth muscle cells 2012-5-8
    Isolation of human prostatic smooth muscle cells Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of

  • 3009 次 Isolation of human primary gastric mucosa epithelial cells 2012-4-19
    Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The

  • 1897 次 Isolation of papillary cells 2012-3-26
    Isolation of papillary cellsIsolation of renal papillary cells1.For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/strept

  • 2129 次 Isolation of lymphatic endothelial cells 2012-3-12
    Dermal Cell Suspensions1.Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery. 2.Dermal sheets were prepared by incubatio

  • 5780 次 Isolation of peripheral blood mononuclear cells (PBMC) 2012-1-16
    Isolation of peripheral blood mononuclear cells (PBMC) 1.Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.PBMC were isolated by centrifugation over lymphoc

  • 3406 次 Stem cell characteristics of amniotic epithelial (AE) cells 2011-12-24
    Stem cell characteristics of amniotic epithelial (AE) cells Isolation of AE Cells1.Human placentae were obtained with the approval of the institutional review board, after uncompli

  • 2448 次 Placental trophoblast and chorionic cell cultures 2011-12-16
    Placental trophoblast and chorionic cell cultures 1.Placental trophoblast and chorionic trophoblast cells were prepared using a modification of the method. 2.Term human placentae a

  • 4022 次 Isolation of human primary ovarian surface epithelial cells 2011-12-12
    Isolation of human primary ovarian surface epithelial cells1.Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institut

  • 5498 次 干細(xì)胞3D scaffold培養(yǎng)技術(shù)的優(yōu)勢(shì)、特色及應(yīng)用范圍 2011-12-5
    3D培養(yǎng)的優(yōu)勢(shì)： 2D細(xì)胞培養(yǎng)時(shí)，細(xì)胞幾乎百分之五十是貼平于培養(yǎng)皿，而另外百分之五十是浸泡在溶液中，最為重要的細(xì)胞與細(xì)胞間接觸卻相當(dāng)?shù)纳�，使得�?xì)胞的生長(zhǎng)形態(tài)與真實(shí)情況差距甚遠(yuǎn)，易造成不

  • 1792 次 Isolation of human prostatic epithelial cells 2011-11-25
    Isolation of human prostatic epithelial cells1.A small piece of tissue from each specimen was removed and minced. 2.The tissue was digested with collagenase overnight. 3.To remove

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